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Estradiol (17β) Mass Concentration in Serum/Plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of estradiol, a key female sex hormone, in your blood. Estradiol helps regulate menstrual cycles, supports reproductive health, and influences bone and cardiovascular health.

Quick summary

["Estradiol levels can help diagnose hormonal imbalances or track treatment for conditions like menopause or fertility issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
47616-8
Short code
Estradiol 1h p ACTH SerPl-mCnc
Synonyms
Estradiol 1h p ACTH SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like irregular periods, breast tenderness, or signs of hormonal disorders. It could also be used during fertility evaluations, before hormone therapy, or to monitor conditions like polycystic ovary syndrome (PCOS).

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the blood draw if your lab requires it. Avoid smoking or drinking alcohol for a few hours beforehand to ensure accurate results.

What Happens During the Test

A healthcare professional will draw blood from a vein in your arm. The sample is then sent to a lab for analysis using a high-performance liquid chromatography (HPLC) method to measure estradiol concentration.

What the Results Mean

Results are typically reported in picograms per milliliter (pg/mL) or similar units. While reference ranges vary by lab, low levels may suggest hormonal deficiencies, while high levels could indicate tumors or other conditions. Your provider will interpret results in the context of your symptoms and medical history.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Estradiol Level

Frequently asked questions

What Does This Test Measure?
This test measures the mass concentration of estradiol, a primary estrogen hormone in women (and smaller amounts in men). Estradiol plays a critical role in reproductive health, bone density, and cardiovascular function. Abnormal levels can signal conditions like ovarian cysts, hormone therapy needs, or menopause-related changes.
How Might My Results Affect My Care?
Your results may guide decisions about fertility treatments, hormone replacement therapy, or the management of conditions like PCOS or thyroid disorders. For example, low estradiol could prompt further testing or treatment adjustments, while very high levels might require investigation for underlying causes like tumors.
How Often Should I Get This Test?
The frequency depends on your medical needs. Your provider may recommend baseline testing during certain life stages (e.g., premenopausal, postmenopausal) or periodically if you’re undergoing hormone-related treatments.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy