Estradiol (Total) by High-Performance Liquid Chromatography (HPLC) with Serum/Plasma Measurement
This test measures the total amount of estradiol, a key female sex hormone, in your blood. Estradiol plays a critical role in reproductive health, bone density, and overall well-being.
["Understanding your estradiol levels can help assess reproductive health and hormonal balance."]
Key facts at a glance
- LOINC
- 56750-3
- Short code
- Estradiol 1h p chal SerPl-mCnc
- Synonyms
- Estradiol 1h p chal SerPl-mCnc
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 20
- Ref high
- 400
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 82670
- SNOMED
- 300919004
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Doctors may order a total estradiol test to evaluate symptoms related to hormonal changes, such as irregular menstrual cycles, infertility, or menopause. It can also be used to monitor hormone replacement therapy or diagnose conditions like polycystic ovary syndrome (PCOS).
What to Expect Before the Test
No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if your blood is drawn early in the morning. Wear comfortable clothing and plan to stay near the testing site for a short time.
How the Test Is Performed
A healthcare provider will draw blood from a vein in your arm using a small needle. You may feel a slight pinch, but the procedure is quick and typically painless. The blood sample is then sent to a lab for analysis using HPLC to measure estradiol levels precisely.
What the Results Mean
Estradiol levels vary depending on your age, menstrual cycle phase (for women), and overall health. Your results will be compared to standard reference ranges provided by your healthcare provider to determine if your levels are within a healthy range or if further evaluation is needed.
Reference range
Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A
For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Estradiol levels help diagnose hormonal imbalances, guide treatment for conditions like low estrogen, and monitor reproductive health. High or low estradiol can indicate underlying issues such as thyroid disorders, tumors, or genetic conditions. Always discuss your results with your doctor for personalized interpretation.
Short name
Total Estradiol
Frequently asked questions
What causes estradiol levels to be tested?
How long does it take to get the results?
Can lifestyle changes affect estradiol levels?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy