Estradiol [Mass/time] in 24-Hour Urine
This test measures the total amount of estradiol, a form of estrogen, excreted in urine collected over 24 hours. It can help clinicians assess estrogen production when a timed urine collection is appropriate.
A 24-hour urine estradiol result reflects estrogen excretion over the entire collection period and must be interpreted alongside symptoms, medications, and other hormone tests.
Key facts at a glance
- LOINC
- 21263-9
- Short code
- Estradiol 24h Ur-mRate
- Synonyms
- Estradiol 24h Ur-mRate
- Category
- chemistry
- Sample
- urine
- Container
- Sterile urine container
- Units
- N/A
- Ref low
- 20
- Ref high
- 400
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 82670
- SNOMED
- 300919004
- Specimen volume
- 10 mL
- Stability
- Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
What Does the Test Measure?
Estradiol is the most abundant form of estrogen in the body. This test measures how much estradiol is eliminated in the urine during a full 24-hour period, rather than testing the blood level at a single moment.
How Is the Urine Collected?
Urine is collected for 24 hours in a labeled container provided by the laboratory or clinic. The collection should begin and end at the specified times, and all urine produced during that period should be included; missed collections or incorrect timing can affect the result.
Why Is This Test Used?
Clinicians may use a 24-hour urine estradiol test to help evaluate estrogen production in selected fertility, endocrine, or hormone-treatment assessments. It may be used with other tests, because estradiol alone may not provide enough information to explain symptoms or treatment response.
What Affects the Result?
Menstrual cycle, pregnancy, menopause, hormone medications, and conditions that alter estrogen production can influence the result. An incomplete collection, unusual urine output, and certain medications may also affect the measured amount.
Reference range
Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A
For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical significance: A 24-hour urine estradiol result can provide information about the body's overall estrogen excretion, but it does not by itself diagnose a specific condition. Interpretation depends on the clinical question, collection quality, medications, and findings from other hormone or imaging tests.
Short name
24-Hour Urine Estradiol
Frequently asked questions
Is this the same as a serum estradiol test?
Do I need to stop taking my medications before this test?
What if I miss part of the 24-hour collection?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy