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24-Hour Urinary Estradiol Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of estradiol, a form of estrogen, collected in your urine over a 24-hour period. It helps assess long-term estrogen levels in your body.

Quick summary

["This test provides insight into your estrogen exposure over time, useful for monitoring hormonal health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
34287-3
Short code
Estradiol 24h Ur-sCnc
Synonyms
Estradiol 24h Ur-sCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms or conditions related to estrogen, such as irregular menstrual cycles, hormonal imbalances, or suspected exposure to estrogenic substances. It can also be used in research or specialized clinical scenarios to evaluate systemic estrogen levels over a full day.

What to Expect Before the Test

No special preparation is needed, but you may be asked to collect all urine for a full 24-hour period. Typically, you’ll start by voiding at a set time (often morning) and then collect all urine until the same time the next day. Store the urine in a cool place or refrigerator until you deliver it to the lab.

What the Test Measures

Estradiol is a primary estrogen hormone in women and a smaller role in men. This test quantifies the total amount of estradiol excreted in your urine over 24 hours, reflecting your body’s ongoing estrogen production and metabolism.

How Results Are Interpreted

Results are not provided with specific reference ranges in this summary, as they vary by lab and individual health factors. Your healthcare provider will interpret the results in the context of your medical history, symptoms, and other tests.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: urine

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Estradiol

Frequently asked questions

What does a 24-hour urine estradiol test tell me about my health?
It provides a comprehensive picture of your body’s estrogen production and metabolism over a full day, which can help identify patterns or imbalances that standard blood tests might miss.
Can I take medications or supplements before this test?
Avoid taking estrogen-containing medications or supplements unless instructed by your provider, as they may affect results. Some over-the-counter supplements or herbal products might also influence hormone levels.
How long will I need to collect urine for this test?
You’ll need to collect all urine for a full 24-hour period, starting from one void (often morning) and continuing until the same time the next day.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy