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24-Hour Urinary Estradiol Rate

Published: August 9, 2026Updated: August 16, 2026

This test measures how much estradiol (a form of estrogen) your body excretes over 24 hours in your urine. It helps assess estrogen levels in your system over time.

Quick summary

["This test helps doctors evaluate long-term estrogen activity, useful for conditions like hormonal imbalances or fertility issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25401-1
Short code
Estradiol 24h Ur-sRate
Synonyms
Estradiol 24h Ur-sRate
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like irregular periods, hormonal imbalances, or if you’re investigating potential estrogen-related conditions. It can also be used to monitor treatment effectiveness for conditions affecting estrogen levels.

What to Expect Before the Test

No special preparation is needed, but you may be asked to collect your urine for 24 hours in a sterile container. Drink plenty of water to help flush out hormones naturally.

How the Test Works

The test measures the total amount of estradiol excreted in your urine over a full day. This provides a more accurate picture of your body’s estrogen production than a single blood test.

What the Results Mean

Results help determine if your estrogen levels are within a healthy range or if they’re too high or too low. Abnormal results may guide further testing or treatment plans.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: urine

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24h Urine Estradiol

Frequently asked questions

What does a low or high result mean?
Low results may suggest hormonal deficiencies, while high results could indicate overproduction of estrogen, which might be linked to conditions like tumors or certain medications. Always discuss results with your doctor.
How often should I get this test?
Your doctor will decide based on your medical history and symptoms. Some patients may need it yearly or more frequently if managing chronic conditions.
Can I take supplements or medications before this test?
Avoid estrogen-containing supplements or medications for at least 2–3 weeks before testing unless instructed otherwise. Always follow your doctor’s specific guidelines.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy