Skip to content

Estradiol (Serum/Plasma) by Immunoassay

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of estradiol, a key female sex hormone, in your blood. Estradiol helps regulate menstrual cycles, supports reproductive health, and influences bone and cardiovascular health.

Quick summary

["Estradiol levels can help diagnose hormonal imbalances or monitor conditions like endometriosis, polycystic ovary syndrome (PCOS), or hormone therapy."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
56749-5
Short code
Estradiol 30M p chal SerPl-mCnc
Synonyms
Estradiol 30M p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to evaluate symptoms such as irregular periods, infertility, or signs of hormonal disorders. It can also be used during pregnancy to monitor fetal development or before starting hormone replacement therapy.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if your lab requires it. Bring any medications you’re currently taking, as some may affect results.

How the Test Works

A blood sample is drawn from a vein in your arm. The sample is then analyzed using an immunoassay method to detect and quantify estradiol levels in your serum or plasma.

What the Results Mean

Results are compared to reference ranges specific to your age, sex, and health status. Low levels may indicate conditions like premature ovarian failure or thyroid disorders, while high levels could suggest tumors or other hormonal issues.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Estradiol Level

Frequently asked questions

How often should I get this test?
The frequency depends on your medical needs. Routine checks may occur annually for women with reproductive health concerns, while pregnant women or those on hormone therapy might need more frequent monitoring.
Can lifestyle changes affect estradiol levels?
Yes, factors like stress, weight fluctuations, and certain medications (e.g., birth control pills) can influence estradiol. Maintaining a balanced diet, managing stress, and avoiding smoking may help stabilize hormone levels.
What if my results are outside the normal range?
If your results are abnormal, your provider will discuss next steps, which may include additional tests, lifestyle adjustments, or treatment options tailored to your condition.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy