Estradiol (17β-Estradiol) by High-Performance Liquid Chromatography (HPLC) with Mass Spectrometry
This test measures the amount of estradiol, a key female sex hormone, in your blood. Estradiol helps regulate menstrual cycles, supports pregnancy, and influences overall reproductive health.
["Understanding your estradiol levels can help assess hormonal balance and reproductive health."]
Key facts at a glance
- LOINC
- 55477-4
- Short code
- Estradiol 4h p chal SerPl-sCnc
- Synonyms
- Estradiol 4h p chal SerPl-sCnc
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 20
- Ref high
- 400
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 82670
- SNOMED
- 300919004
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why Is This Test Done?
Your healthcare provider may order this test to evaluate symptoms like irregular periods, infertility, or hormonal imbalances. It can also be used during pregnancy to monitor fetal development or in menopause to diagnose hormone-related conditions.
What to Expect Before the Test
No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the blood draw if instructed. Wear comfortable clothing and plan to rest afterward, as you might feel lightheaded.
What Happens During the Test?
A healthcare professional will insert a small needle into a vein in your arm to collect a blood sample. You may feel a brief pinch, but the procedure is quick and typically painless. The blood is then sent to a lab for analysis.
What Are the Results Measured Against?
Results are compared to standard ranges for your age, sex, and health status. These ranges can vary, so your results will be interpreted by your doctor based on your specific needs.
Reference range
Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A
For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance: How Does Estradiol Affect Health?
Short name
Estradiol Level (Serum)
Frequently asked questions
Low estradiol levels may indicate conditions like premature menopause, ovarian dysfunction, or certain cancers, while high levels could signal polycystic ovary syndrome (PCOS) or tumors. Monitoring estradiol helps guide treatments for hormonal disorders.
If your estradiol level is too low, your doctor may recommend hormone therapy, lifestyle changes, or further testing to identify the cause. If it’s too high, they might investigate conditions like PCOS or tumors and adjust treatment plans accordingly.
The frequency depends on your symptoms and medical history. Your doctor will determine how often to check your estradiol levels, often every few months or as needed for monitoring.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy