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Estradiol and Creatinine, 24-Hour Urine

Published: August 9, 2026Updated: August 16, 2026

Measures estradiol and creatinine collected over 24 hours. Creatinine may be used to help account for variations in urine concentration.

Quick summary

["This test measures estrogen production over a full day using a 24-hour urine collection."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
13736-4
Short code
Estradiol/Creat 24h Ur
Synonyms
Estradiol/Creat 24h Ur
Category
kidney
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures the amount of estradiol, a form of estrogen, in urine collected for 24 hours. It may also measure creatinine, a waste product used to help assess urine concentration.

Why might I need this test?

A 24-hour urine estradiol test may be used to evaluate estrogen production when the clinical situation requires measurement over a full day. It may be considered alongside other tests when assessing hormone function or monitoring certain treatments.

How is the test performed?

You collect all urine for exactly 24 hours, usually from the time you begin collection until the same time the following day. The laboratory then measures estradiol and creatinine in the collection, and the results may be reported as a concentration or a ratio.

How do I prepare for the test?

Follow the laboratory's collection instructions carefully, including the start and end times, container type, and any storage or refrigeration requirements. Tell your clinician about pregnancy, your menstrual cycle, and medicines or supplements that could affect hormone results.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: urine

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urine Estradiol

Frequently asked questions

Why is creatinine included with urine estradiol?
Creatinine helps show how concentrated the urine sample is. This can help account for differences in urine concentration when interpreting the estradiol measurement.
How is a 24-hour urine collection completed?
Collect all urine during the full 24-hour period, including any urine produced at night, and return the container as instructed. Missing urine or extending the collection beyond the specified time can make the result less reliable.
Can medications or pregnancy affect the result?
Yes. Hormonal medicines, some other medications, pregnancy, and menstrual-cycle changes may affect estradiol levels. Tell the ordering clinician about these factors before interpreting the result.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy