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Estradiol, Serum (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of estradiol, a form of estrogen, in your blood serum. Estradiol is a key hormone in women’s reproductive health and plays a role in regulating menstrual cycles, bone density, and breast development.

Quick summary

["Estradiol helps balance hormones and supports overall health, especially in women."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
35384-7
Short code
Estradiol SerPl HS-mCnc
Synonyms
Estradiol SerPl HS-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
high-sensitivity
CPT
82670
SNOMED
300919004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to evaluate hormone-related conditions, such as irregular menstrual cycles, infertility, or symptoms of perimenopause/menopause. It can also help diagnose or monitor conditions like ovarian cysts, polycystic ovary syndrome (PCOS), or hormone-related cancers.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if your blood is drawn early in the morning. Avoid smoking or caffeine for a few hours before testing, as they can affect hormone levels.

What Happens During the Test?

A healthcare professional will draw blood from a vein in your arm. The blood sample is sent to a lab for analysis using a sensitive immunoassay method to measure estradiol concentration.

What Are the Results Mean?

Results are compared to a range of typical values for your age and sex. Abnormal results may indicate hormonal imbalances, reproductive health concerns, or other conditions requiring further evaluation.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Estradiol Level

Frequently asked questions

How long will it take to get my results?
Results are typically available within 1–3 business days, depending on your lab’s processing time. Follow up with your healthcare provider to discuss the results and any next steps.
Can lifestyle changes affect estradiol levels?
Yes, factors like diet, exercise, stress, and certain medications can influence estradiol levels. Avoiding smoking, managing stress, and maintaining a balanced diet may support stable hormone levels.
Is this test the same as a pregnancy test?
No, this test measures estradiol, a form of estrogen unrelated to pregnancy. Pregnancy tests detect hCG hormone instead.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy