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Estradiol, Serum (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of estradiol, a key female sex hormone, in your blood serum. Estradiol helps regulate menstrual cycles, supports reproductive health, and influences bone and breast tissue.

Quick summary

["Estradiol levels can help diagnose hormonal imbalances or track treatment effectiveness."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
2243-4
Short code
Estradiol SerPl-mCnc
Synonyms
Estradiol SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to evaluate symptoms like irregular periods, infertility, or signs of hormonal disorders. It can also be used during pregnancy monitoring, before surgery (e.g., hysterectomy), or to assess hormone replacement therapy.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the draw if instructed. Wear comfortable clothing for easy access to your vein. Bring any medications or supplements you’re taking, as they may affect results.

How the Test Is Performed

A healthcare professional will draw blood from a vein in your arm using a small needle. You may feel a brief pinch, but the procedure is quick. The blood sample is sent to a lab for analysis.

What the Results Mean

Results are compared to a general reference range, but individual ranges can vary by lab. High or low levels may signal conditions like polycystic ovary syndrome (PCOS), thyroid disorders, or menopause-related changes. Your provider will discuss findings in context with your symptoms.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Serum Estradiol Level

Frequently asked questions

What Are Common Causes of Abnormal Results?
Elevated estradiol levels may indicate conditions like ovarian tumors, early pregnancy, or certain medications. Low levels could suggest menopause, pituitary disorders, or hormonal therapy side effects. Always consult your provider for personalized interpretation.
How Often Should I Get This Test?
Frequency depends on your health needs. Routine checks may occur during fertility evaluations, pre-surgery planning, or if you’re on hormone treatments. Follow your provider’s recommendations.
Can I Avoid This Test?
If you have no symptoms or medical reasons, you may not need this test. However, if your provider suspects hormonal imbalances, they’ll determine if testing is necessary.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy