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Estradiol (serum/plasma) by immunoassay (confirmation)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of estradiol, a key female sex hormone, in your blood. It helps confirm levels after initial screening.

Quick summary

["Confirming estradiol levels helps diagnose hormonal imbalances or monitor treatment for conditions like endometriosis, menopause, or fertility issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
58492-0
Short code
Estradiol sp1 p chal SerPl HS-mCnc
Synonyms
Estradiol sp1 p chal SerPl HS-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
high-sensitivity
CPT
82670
SNOMED
300919004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like irregular periods, breast tenderness, or signs of hormonal changes. It’s also used to monitor hormone therapy or evaluate conditions like polycystic ovary syndrome (PCOS) or ovarian dysfunction.

What to Expect Before the Test

No special preparation is needed, but avoid smoking or drinking alcohol 24 hours before the draw. If you’re on hormone therapy, check with your provider about timing.

What the Results Mean

Results help determine if your estradiol levels are within expected ranges for your age and health status. Abnormal results may signal conditions requiring further evaluation.

Common Conditions Monitored

This test supports diagnosis of hormonal disorders, fertility issues, or menopause-related symptoms. It’s also used in pre- and postmenopausal care.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical significance: Estradiol levels guide treatment decisions for conditions like endometriosis, ovarian cysts, or hormone replacement therapy. Low or high levels may indicate underlying health concerns that need medical attention.

Short name

Estradiol Confirmation

Frequently asked questions

How often should I get this test?
Frequency depends on your health needs. Your provider will determine how often based on symptoms, medical history, or treatment plans.
What if my results are outside the normal range?
If results are abnormal, your provider will discuss next steps, which may include additional tests or adjustments to your treatment plan.
Can diet or supplements affect estradiol levels?
Certain foods, supplements, or medications can influence hormone levels. Always inform your provider about all supplements or dietary changes before testing.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy