Estradiol (17β) in Serum or Plasma by High-Performance Liquid Chromatography (HPLC)
This test measures the amount of estradiol, a primary female sex hormone, in your blood. It helps assess hormone balance, reproductive health, and potential conditions like hormonal imbalances or fertility issues.
["Estradiol levels can indicate reproductive health and hormone-related conditions."]
Key facts at a glance
- LOINC
- 58489-6
- Short code
- Estradiol sp4 p chal SerPl HS-mCnc
- Synonyms
- Estradiol sp4 p chal SerPl HS-mCnc
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 20
- Ref high
- 400
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- high-sensitivity
- CPT
- 82670
- SNOMED
- 300919004
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Your healthcare provider may order this test to evaluate symptoms such as irregular menstrual cycles, menopause-related changes, or to monitor hormone therapy. It can also help diagnose conditions like polycystic ovary syndrome (PCOS) or evaluate fertility.
What to Expect Before the Test
No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if serum/plasma is required. Avoid smoking or consuming alcohol for a few hours beforehand. Bring any medications you’re currently taking to your appointment.
How the Test Is Performed
A blood sample will be drawn from a vein in your arm by a healthcare professional. The blood is then processed and analyzed using high-performance liquid chromatography (HPLC) to measure estradiol levels.
What the Results Mean
Results are typically reported as a mass concentration of estradiol in your blood. While reference ranges vary by lab and age, lower or higher than expected levels may signal hormonal imbalances or other health concerns that need further evaluation.
Reference range
Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A
For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance: Estradiol plays a critical role in reproductive health, bone density, and cardiovascular function. Abnormal levels may indicate conditions like ovarian dysfunction, thyroid disorders, or early menopause. Regular monitoring is important for women undergoing hormone therapy or managing symptoms of perimenopause.
Short name
Estradiol Level
Frequently asked questions
What causes estradiol levels to be tested?
How long does it take to get results?
Can lifestyle changes affect estradiol levels?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy