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Estradiol (Serum/Plasma) by Immunoassay (Confirmatory)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of estradiol, a key female sex hormone, in your blood. It helps assess hormone balance, fertility, or symptoms like irregular periods, menopause, or related conditions.

Quick summary

["Estradiol levels can guide treatment for hormonal imbalances or reproductive health concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
51497-6
Short code
Estradiol sp5 p chal SerPl-mCnc
Synonyms
Estradiol sp5 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
SNOMED
300919004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order estradiol testing if you experience symptoms linked to hormonal changes, such as irregular menstrual cycles, hot flashes, or fertility issues. It may also be used to monitor hormone therapy or evaluate conditions like polycystic ovary syndrome (PCOS) or thyroid disorders.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the draw if your lab requires it. Bring your lab results or a list of medications to your appointment.

How the Test Is Performed

A healthcare provider will collect a blood sample from a vein in your arm using a small needle. You may feel a brief pinch, but the procedure is quick and usually painless.

What the Results Mean

Results help doctors compare your hormone levels to typical ranges for your age and sex. Abnormal levels may signal conditions like hormone deficiencies, tumors, or other health concerns that need further evaluation.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Estradiol Levels and Health

Short name

Estradiol Level (Confirm)

Frequently asked questions

How long will I have to wait for my results?
Results are typically available within 1–3 business days, depending on your lab. Some labs may provide preliminary results sooner, but confirmatory tests like this often take longer due to additional testing steps.
What does a low or high estradiol level indicate?
Low estradiol levels may suggest menopause, ovarian dysfunction, or thyroid disorders, while high levels could indicate conditions like ovarian cysts, tumors, or hormonal therapy use. Your doctor will interpret results in the context of your symptoms and medical history.
Can I take anything to adjust my estradiol levels before the test?
Avoiding certain medications (like birth control or hormone treatments) may be advised by your doctor, but they won’t alter the test itself. Follow your provider’s instructions if they recommend changes.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy