24-Hour Urine Collection for Total Estrogen (Substance Concentration)

Why This Test Is Done

Your healthcare provider may order this test if they suspect hormonal imbalances, such as low or high estrogen levels, or to monitor conditions like endometriosis, ovarian dysfunction, or certain cancers. It can also help evaluate symptoms like irregular periods, infertility, or menopausal changes.

How the Test Works

You’ll need to collect all urine produced in a 24-hour period, typically starting after midnight one day and ending after midnight the next. The urine is then sent to a lab for analysis to determine the total estrogen concentration.

Preparation and Collection

Follow your healthcare provider’s instructions carefully. You’ll need a clean container to collect all urine, including the first and last voids. Drink plenty of water to help flush out hormones, but avoid alcohol and certain medications that might affect results.

What to Expect After the Test

After collection, the urine sample is processed and analyzed in a lab. Results are usually reported as a total amount of estrogen excreted over 24 hours, though exact reference ranges vary by lab. You’ll receive a report from your provider with the results and any next steps.