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Total Protein with Albumin/GLOBulin Separation (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures your blood’s total protein level and separates it into albumin and globulin fractions, which help assess liver function, nutritional status, and immune health.

Quick summary

["This test helps identify potential liver issues, protein malnutrition, or immune system problems."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94469-4
Short code
F2 20210G>A + F5 1691G>A Pnl Bld/T
Synonyms
F2 20210G>A + F5 1691G>A Pnl Bld/T
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
molgen
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters in Your Health

Your body’s proteins, including albumin and globulins, play key roles in maintaining fluid balance, supporting immune function, and transporting nutrients. Abnormal levels may signal conditions like liver disease, kidney problems, or malnutrition.

How the Test Works

A small blood sample is drawn and analyzed to measure total protein concentration and break it down into albumin (the protein that keeps fluids in your blood vessels) and globulin (proteins involved in immunity and clotting).

What to Expect Before the Test

No special preparation is needed, but inform your provider if you’re taking blood thinners or have recent injuries. Wear comfortable clothing for easy access to your arm.

Common Conditions Monitored

This test helps diagnose or monitor conditions like cirrhosis (liver scarring), chronic kidney disease, malnutrition, or autoimmune disorders where protein levels may be affected.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical significance: Elevated total protein or globulins could indicate inflammation, infections, or certain cancers, while low levels may signal liver disease, kidney failure, or poor nutrition. Albumin specifically is often checked for signs of fluid retention or liver dysfunction.

Short name

Total Protein Profile

Frequently asked questions

What does a ‘normal’ result mean for me?
A ‘normal’ result doesn’t mean you’re healthy—it just means your protein levels fall within the expected range for your age and health. Your provider will use these results alongside other tests to guide care.
Will I need to fast before this test?
No fasting is required, but you may be asked to avoid certain medications (like blood thinners) or foods that could affect protein levels (like high-protein supplements) if instructed by your provider.
Can I take this test at home?
No, this test requires a blood draw in a clinical setting. Some labs may offer at-home blood collection services, but results will still be processed in a lab.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy