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Factor I (Activated Clotting Time) – Plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures how long it takes for blood plasma to clot after activation, helping assess blood clotting function.

Quick summary

["ACT helps diagnose bleeding or clotting disorders by tracking how quickly your blood forms a clot under controlled conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
96455-1
Short code
Fact II Act + Inhib Pnl PPP
Synonyms
Fact II Act + Inhib Pnl PPP
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
coag
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is ACT Used For?

ACT is primarily used in medical settings to monitor blood clotting during procedures like cardiopulmonary bypass, dialysis, or when evaluating patients with suspected bleeding or clotting disorders. It provides a quick baseline measurement of clotting efficiency in plasma.

How Is the Test Performed?

A small sample of your blood is drawn into a specialized tube containing a clotting activator. The blood is then placed in a device that measures the time it takes for a clot to form. This process is typically done in a clinical lab under sterile conditions.

Sample Requirements and Preparation

For this test, you’ll need a blood sample collected in a special tube. No special preparation is required, but fasting may be advised in some cases depending on the clinical context (e.g., monitoring anticoagulant therapy).

What Does an Abnormal ACT Indicate?

An ACT that is too long may suggest impaired clotting (e.g., due to bleeding disorders, low levels of clotting factors, or medications like anticoagulants). An ACT that is too short may indicate hypercoagulability or laboratory contamination. Results are interpreted in relation to clinical symptoms and other lab tests.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

ACT (Activated Clotting Time)

Frequently asked questions

Why might my doctor order an ACT?
Your doctor may order an ACT to evaluate blood clotting function before or during certain medical procedures, such as surgery, dialysis, or cardiopulmonary bypass. It can also help diagnose bleeding disorders, assess anticoagulant therapy, or monitor patients with suspected clotting issues.
What are the typical reference ranges for ACT?
ACT reference ranges vary by lab and clinical context. Generally, a normal ACT is around 15 to 25 seconds, but this can differ based on the specific method and patient condition. Your results will be compared to your individual baseline and clinical picture.
Can I influence my ACT results?
While you cannot directly alter your ACT results, certain medications (like blood thinners), dietary supplements, or medical conditions (such as liver disease) may affect clotting. Avoiding certain medications or supplements before testing may help ensure accurate results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy