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Serum or plasma concentration of free fatty acids (FFAs) measured by immunoassay

Published: August 9, 2026Updated: August 16, 2026

This test measures the levels of free fatty acids, which are important fatty molecules released from fat cells into the bloodstream. They play a key role in energy production and metabolism.

Quick summary

["Free fatty acids help your body use energy efficiently and support normal metabolic function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
3235-9
Short code
Fact XII Ag Act/Nor PPP IA
Synonyms
Fact XII Ag Act/Nor PPP IA
Category
chemistry
Sample
plasma
Container
Lavender-top (EDTA) or Green-top (Heparin)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may order this test to evaluate metabolic health, assess conditions affecting fat metabolism, or monitor treatment responses in conditions like diabetes, thyroid disorders, or liver disease.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if it involves a blood draw. Avoid excessive alcohol or strenuous activity 24 hours prior.

How the Test Works

A small blood sample is taken from a vein in your arm. The sample is then analyzed using an immunoassay method to measure the concentration of free fatty acids in your serum or plasma.

What the Results Mean

Results help identify imbalances in fat metabolism, which may indicate conditions like hyperlipidemia, malnutrition, or hormonal imbalances. Your provider will interpret results in the context of your overall health.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Elevated free fatty acid levels may signal metabolic disorders, such as uncontrolled diabetes or thyroid dysfunction, while low levels could suggest malnutrition, liver disease, or certain endocrine conditions. Regular monitoring is important for patients with metabolic disorders or those undergoing treatment for fat metabolism issues.

Short name

Free Fatty Acids (serum/plasma)

Frequently asked questions

What causes free fatty acid levels to change?
Several factors can influence free fatty acid levels, including diet (e.g., high-fat meals), physical activity, hormonal changes (like those during pregnancy or thyroid disorders), and underlying conditions such as diabetes or liver disease. Stress, illness, or certain medications may also affect them.
How often should I get this test?
Your provider will determine the frequency based on your health needs. For example, people with diabetes or metabolic disorders may need this test more often than others. Follow their guidance for scheduling.
Can lifestyle changes affect my test results?
Yes, diet, exercise, and weight management can impact free fatty acid levels. Reducing high-fat foods, staying hydrated, and maintaining a balanced diet may help stabilize your results. Always discuss lifestyle changes with your healthcare provider first.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy