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Folate in Serum (or Plasma) by High-Performance Liquid Chromatography (HPLC)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of folate, a B vitamin important for DNA synthesis and red blood cell production, in your blood.

Quick summary

["Folate helps prevent birth defects and supports overall cell function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
1386-2
Short code
FCA Fract 2h p 300 ug CLN PO Plas-mCnc
Synonyms
FCA Fract 2h p 300 ug CLN PO Plas-mCnc
Category
chemistry
Sample
plasma
Container
Lavender-top (EDTA) or Green-top (Heparin)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to check for folate deficiency, which can cause anemia, neurological issues, or increased risk of certain cancers. It’s also used in pregnancy to assess folate levels and prevent neural tube defects in the baby.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if your lab uses a different method. Bring any medications you’re taking, as some (like certain antacids) can affect results.

What Happens During the Test?

A blood sample is drawn from a vein, usually in your arm. The sample is sent to a lab for analysis using HPLC to measure folate levels precisely.

What Do the Results Mean?

Low folate levels may indicate poor diet, malabsorption issues, or increased needs (e.g., pregnancy). High levels might suggest folate supplements or certain medical conditions. Your provider will interpret results in the context of your health.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Serum Folate

Frequently asked questions

How long does it take to get my results?
Results are typically available within 1–3 business days, depending on your lab’s processing time. Follow-up may be scheduled if your levels are outside expected ranges.
Can I eat or drink anything before this test?
Standard fasting guidelines apply, but confirm with your lab or provider about specific instructions for your test method. Some labs may allow water.
Will this test affect my medications?
Folate supplements or certain medications (like antacids) can alter results. Always inform your provider about all medications, including over-the-counter items.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy