Serum or plasma protein electrophoresis with bromocresol green and bromocresol purple staining

What This Test Measures

Protein electrophoresis separates blood proteins into bands based on size and electrical charge. Bromocresol green and purple dyes highlight specific protein groups, including albumin, globulins, and other fractions. This helps detect imbalances that might indicate conditions like chronic inflammation, infections, or genetic disorders.

Common Uses and Conditions

This test is commonly used to diagnose or monitor conditions such as multiple myeloma (a cancer of plasma cells), liver disease, kidney disease, and certain autoimmune disorders. It may also be used to evaluate protein malnutrition or to assess the effectiveness of treatments.

Sample Preparation and Testing Process

A blood sample is drawn, and the serum or plasma is separated. The sample is then run through an electric field to separate proteins, which are visualized using the bromocresol dyes. The results show distinct bands representing different protein types.

What the Results Mean

Interpretation depends on the pattern of protein bands. For example, an increased amount of a specific protein band may suggest a particular disease or condition. Your healthcare provider will compare the results to your medical history and other tests to determine the next steps.