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Respiratory SARS-CoV-2 Nucleic Acid Detection (Qualitative) via Reverse Transcription Polymerase Chain Reaction (RT-PCR)

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence of genetic material from the virus that causes COVID-19 in your respiratory sample. It helps determine if you may be infected and could spread the virus.

Quick summary

["A COVID-19 test to identify active infection by detecting the virus’s genetic material in your nose or throat."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
95423-0
Short code
FLUABV + SARS-CoV-2 Resp NAA+probe
Synonyms
FLUABV + SARS-CoV-2 Resp NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

The test uses a small swab to collect a sample from your upper respiratory tract (nose or throat). The swab is then sent to a lab where it undergoes a process called reverse transcription polymerase chain reaction (RT-PCR). This method amplifies and detects specific genetic sequences unique to SARS-CoV-2, the virus responsible for COVID-19. If the virus is present, the test will identify it.

Sample Collection

You will receive a swab to gently insert into your nose or throat. The process is quick and usually painless. You may experience a slight discomfort or mild irritation, but it is temporary. Follow your healthcare provider’s instructions carefully to ensure proper sample collection.

When You Might Need This Test

This test is commonly used when you have symptoms of COVID-19, such as fever, cough, shortness of breath, or fatigue. It may also be recommended if you have been exposed to someone with COVID-19, or if you are required for travel, employment, or other reasons by public health guidelines.

Test Results and Interpretation

Results are typically reported as either positive (indicating the presence of the virus) or negative (indicating no detectable virus). A positive result means you may be infected and could spread the virus to others. A negative result does not necessarily mean you are not infected, especially if you have symptoms or were exposed recently. Follow-up testing or monitoring may be advised by your healthcare provider.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

SARS-CoV-2 RT-PCR Test

Frequently asked questions

What does a positive test mean?
A positive test result means that the test detected genetic material from the virus that causes COVID-19 in your sample. This suggests you may be infected and could be contagious. It is important to isolate yourself from others to prevent spreading the virus.
Can I get this test at home?
Some over-the-counter tests (often called antigen tests) can be done at home. However, the test described here is typically performed in a clinical setting by a healthcare provider or at a testing facility. These tests are more sensitive and are used to confirm infection.
How long does it take to get results?
Results from this type of test are usually available within 24 to 48 hours, depending on the lab’s processing time. Some facilities may offer same-day results, especially during high-demand periods.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy