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Anti-Human Immunodeficiency Virus (HIV) Antibody Screening by Immunofluorescence Assay (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test detects antibodies to HIV, the virus that causes AIDS, in your blood or plasma. It helps determine if you’ve been exposed to the virus.

Quick summary

["An HIV antibody test checks for past or recent infection; results don’t confirm active disease without further testing."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
80590-3
Short code
FLUAV H3 HA gene Nph Ql NAA+probe
Synonyms
FLUAV H3 HA gene Nph Ql NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may recommend this test if you have risk factors for HIV, such as unprotected sex, shared needles, or a history of sexually transmitted infections. It’s also part of routine screening for certain groups (e.g., pregnant women, people with frequent blood tests).

How the Test Works

The test uses a fluorescent antibody method to identify HIV-specific proteins in your blood or plasma. A positive result means your immune system has mounted an antibody response, possibly indicating past exposure.

What to Expect Before the Test

No special preparation is needed. You’ll provide a blood sample (usually from a vein) or plasma (from a tube) at a lab. Avoid alcohol for 24 hours before testing if instructed.

What to Expect After the Test

Results are typically ready within 24–72 hours. You’ll discuss them with your provider, who may order follow-up tests (e.g., HIV viral load) for confirmation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

A positive result requires further testing (e.g., Western blot or PCR) to confirm HIV infection. Early detection allows access to treatment, which can slow disease progression. A negative result means no detectable antibodies at the time of testing—re-testing may be needed if you’ve had recent exposure.

Short name

HIV Antibody Screen (IFIA)

Frequently asked questions

How soon after exposure should I get tested?
HIV antibodies may take 2–12 weeks to develop after exposure. Some tests can detect earlier (window period), but results may still be inconclusive. Ask your provider about timing based on your risk factors.
Can I have false-positive or false-negative results?
False positives are rare but possible due to cross-reactions with other viruses. False negatives can occur if tested too early after exposure. If results are unclear, repeat testing or additional tests will be needed.
Does this test show if I have active HIV infection?
No. This test only detects antibodies to HIV. Active infection requires confirmation with tests that measure the virus’s presence (e.g., viral load). Always discuss results with your healthcare provider.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy