Influenza Virus Nucleic Acid Sequence Detection (Qualitative) by Real-Time Polymerase Chain Reaction (RT-PCR)

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like fever, cough, sore throat, body aches, or fatigue. It’s also used to confirm flu diagnosis in patients with suspected infection, especially during flu season or outbreaks. Some settings may also use it for screening before certain procedures or in high-risk groups.

How the Test Works

The test uses a small sample of your respiratory secretions (like nasal swab or throat swab) to detect the virus’s genetic material. A lab technician extracts DNA/RNA from the sample and amplifies it using RT-PCR, which creates many copies of the virus’s genetic material for detection. If the virus is present, the test will identify it.

Sample Collection and Preparation

You’ll likely have a quick nasal or throat swab. The swab collects cells and fluids from your upper respiratory tract, which are then sent to a lab for testing. No special preparation is needed, but avoid smoking or using nasal sprays before the test if instructed.

Test Results and Interpretation

Results are qualitative (positive or negative) and indicate whether the virus is detected in your sample. A positive result means the flu virus is likely present, while a negative result suggests it’s not detected at the time of testing. Follow-up testing or additional symptoms may be needed for confirmation.