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Fluorescent Immunoassay for Anti-Nuclear Antibodies (ANA) in Serum

Published: August 9, 2026Updated: August 16, 2026

This test measures antibodies that may indicate certain autoimmune conditions by detecting the presence of anti-nuclear antibodies in your blood.

Quick summary

["Helps identify potential autoimmune diseases by checking for abnormal antibodies targeting your body’s cells."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
50705-3
Short code
FLUAV NS RNA Islt Ql Seq
Synonyms
FLUAV NS RNA Islt Ql Seq
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
sequencing
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Is an ANA Test Used For?

ANA tests are often used as a screening tool to check for autoimmune diseases, such as lupus, scleroderma, or rheumatoid arthritis. They can help doctors evaluate symptoms like joint pain, fatigue, or unexplained rashes by identifying whether your immune system is attacking your own tissues.

How Is the ANA Test Performed?

A small blood sample is drawn from your arm. The serum (liquid part of blood) is then tested using a fluorescent immunoassay. This method detects antibodies that bind to nuclear parts of cells, producing a fluorescent signal if they are present.

What Might Cause an ANA Test to Be Ordered?

Your doctor may recommend this test if you have symptoms like joint swelling, unexplained fatigue, fever, or skin rashes. It’s also used in follow-up testing for known autoimmune conditions to monitor disease activity.

What Does a Positive ANA Test Mean?

A positive result means antibodies were found, which may suggest an autoimmune condition. However, a positive ANA does not always mean you have a specific disease—many healthy people test positive. Further testing is often needed to determine the exact cause.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

ANA Fluorescence Test

Frequently asked questions

Why might my doctor want to repeat this test?
If your initial ANA test was positive, your doctor may order follow-up tests (like specific antibody tests) to narrow down the possible conditions. They may also check for other symptoms or perform additional bloodwork to confirm or rule out autoimmune diseases.
Can lifestyle changes affect ANA test results?
ANA test results are primarily about detecting antibodies, not directly influenced by diet or exercise. However, managing symptoms of autoimmune diseases (like rest, stress reduction, or medications) can help your doctor assess overall health better.
What if my ANA test is negative but I still have symptoms?
A negative ANA test means no antibodies were detected in this test, but it doesn’t rule out all autoimmune conditions. Other tests or symptoms may be needed to diagnose your specific issue. Always discuss your concerns with your doctor.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy