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Fluorohemoglobin (HbF) by High-Performance Liquid Chromatography (HPLC) in Serum

Published: August 9, 2026Updated: August 16, 2026

This test measures the level of fetal hemoglobin (HbF) in your blood, a type of hemoglobin found in babies and some adults with certain blood disorders.

Quick summary

["HbF levels can help diagnose or monitor conditions like thalassemia or sickle cell disease."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
6640-7
Short code
FLUAV Phill Ab Ser HAI-aCnc
Synonyms
FLUAV Phill Ab Ser HAI-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
hai
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

HbF is a form of hemoglobin that carries oxygen in your blood. In newborns, it makes up most of the hemoglobin, but as you grow, it decreases. Certain genetic conditions can cause higher levels of HbF in adults. This test specifically uses HPLC to separate and measure HbF from other hemoglobin types.

Why Might You Need This Test?

Your healthcare provider may order this test if you have symptoms of blood disorders, such as fatigue, pale skin, or frequent infections, or if you have a family history of hemoglobin-related conditions. It may also be used during pregnancy to screen for fetal hemoglobin levels.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The blood is then processed to separate the serum, which is sent to the lab for testing via HPLC. This method helps accurately identify HbF levels.

What to Expect Before the Test

No special preparation is needed for this test. You can eat and drink normally on the day of your test. If you are pregnant, inform your healthcare provider before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

HbF (Serum)

Frequently asked questions

What does an abnormal HbF level mean?
An abnormal HbF level could suggest an underlying genetic blood disorder. Your healthcare provider will discuss the results with you and may recommend further testing or treatments based on your symptoms and test findings.
How is HbF tested?
HbF is tested using High-Performance Liquid Chromatography (HPLC), a method that separates hemoglobin types based on their structure and measures their concentration in your blood serum.
Can HbF levels change over time?
Yes, HbF levels can vary depending on age, health conditions, and genetic factors. For example, newborns have higher HbF levels, which gradually decrease as they grow. Certain blood disorders can cause persistent changes in HbF levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy