Fluorescent Immunoassay for Antigen Detection in Lower Respiratory Specimen (e.g., sputum or induced sputum)

How the Test Works

A sample of your mucus (sputum) is collected from your lower respiratory tract, often by coughing into a container or using a device to induce sputum. The sample is then tested using a fluorescent immunoassay, which detects specific antigens (proteins) from viruses or bacteria. The test may appear qualitative (positive/negative) or semi-quantitative depending on the pathogen.

What to Expect Before the Test

You may be asked to rinse your mouth with water and avoid coughing or swallowing the mucus sample. Some tests may require you to cough deeply into a sterile container or use a specialized device to collect mucus from your lungs. Follow your healthcare provider’s instructions carefully.

Common Uses of This Test

This test is often used to diagnose respiratory infections, such as influenza, respiratory syncytial virus (RSV), or other viral/bacterial pathogens in patients with symptoms like coughing, fever, or difficulty breathing. It can also help guide treatment decisions.

Sample Collection Process

The sample is typically collected by coughing into a sterile container or using a device that helps bring mucus from your lungs to the surface. If needed, your provider may instruct you to take deep breaths or use a saline rinse to loosen mucus before collecting the sample.