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Red Blood Cell (RBC) Hemolysis Profile (Frozen Sample)

Published: August 9, 2026Updated: August 17, 2026

This test evaluates the presence of free hemoglobin in frozen red blood cell samples, which can indicate red blood cell destruction (hemolysis).

Quick summary

["Hemolysis may signal underlying conditions like autoimmune disorders or infections."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
54422-1
Short code
Frozen RBC ped Gvn BPU
Synonyms
Frozen RBC ped Gvn BPU
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
4.2
Ref high
5.9
Critical range
2 N/A – 7 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85041
SNOMED
900000000000003001
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like fatigue, pale skin, dark urine, or signs of anemia. It’s also used to monitor conditions affecting red blood cells, such as sickle cell disease or certain infections.

What to Expect Before the Test

No special preparation is needed. Blood is drawn into a tube containing an anticoagulant, which prevents clotting. The sample is then frozen for testing.

How the Test Works

The frozen sample is analyzed to detect free hemoglobin, a breakdown product of red blood cells. Elevated levels suggest hemolysis, where red blood cells are breaking down prematurely.

Possible Results and Next Steps

If results are abnormal, further testing may be needed to identify the cause, such as blood tests for specific antibodies or infections. Follow-up with your provider to discuss next steps.

Reference range

Adult males: 4.2-5.9; Females: 3.8-5.1 x 10^12/LN/A

Specimen: serum

No special preparation required. Stay well hydrated.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

RBC Hemolysis Screening

Frequently asked questions

What causes hemolysis in frozen samples?
Freezing can sometimes trigger artifactual hemolysis due to cell membrane damage or chemical changes. However, this test specifically measures free hemoglobin to assess true hemolysis, not artifacts.
How long does it take to get results?
Results may take several days to weeks, depending on lab processing and frozen sample handling. Your provider will contact you once available.
Can I prevent hemolysis in blood samples?
While you can’t prevent hemolysis entirely, proper handling (e.g., avoiding excessive movement) and using anticoagulants help maintain sample integrity. Follow your lab’s instructions for storage.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy