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Follicle Stimulating Hormone (FSH) with Triple Bolus Testing in Serum/Plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures Follicle Stimulating Hormone (FSH), a hormone produced by the pituitary gland that plays a key role in reproductive health. The triple bolus method helps assess FSH levels over time to better understand hormone function.

Quick summary

["FSH levels can help diagnose fertility issues, hormone imbalances, or other reproductive health conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
40361-8
Short code
FSH 1h p Triple Bolus SerPl-aCnc
Synonyms
FSH 1h p Triple Bolus SerPl-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.8
Ref high
1.2
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85610
SNOMED
396252006
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Performed

Your doctor may order this test if you have symptoms like irregular menstrual periods, difficulty becoming pregnant, or signs of hormonal imbalances. It may also be used to monitor conditions like polycystic ovary syndrome (PCOS) or evaluate pituitary gland function.

What to Expect Before the Test

No special preparation is needed. Blood will be drawn from a vein, usually in your arm. You may feel a slight pinch, but it typically doesn’t cause discomfort. If you’re on blood thinners, let your doctor know before the test.

How the Test Works

The triple bolus method involves drawing blood at three different times to measure FSH levels under different conditions. This helps provide a clearer picture of how your body responds to hormonal signals.

What the Results Mean

Results are interpreted by your healthcare provider based on your symptoms, medical history, and other test results. Abnormal levels may indicate conditions affecting fertility, hormone production, or reproductive health.

Reference range

INR: 0.8-1.2 (warfarin: 2.0-3.0)N/A

Specimen: serum

Inform your healthcare provider if you are taking blood thinners. No special preparation required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: FSH plays a critical role in regulating the menstrual cycle and sperm production. Elevated or low levels can signal potential issues with ovulation, ovarian function, or pituitary disorders, guiding treatment decisions for fertility or endocrine conditions.

Short name

FSH Triple Bolus Test

Frequently asked questions

What does a triple bolus test add compared to a single FSH measurement?
A triple bolus test provides more detailed timing information about FSH fluctuations, which can help identify patterns or abnormalities in hormone release that a single measurement might miss.
Can lifestyle factors like diet or stress affect FSH levels?
Yes, stress, poor sleep, and certain dietary habits may influence hormone levels, including FSH. However, the triple bolus test focuses specifically on measuring FSH activity over time rather than overall lifestyle impact.
How soon will I get my results, and what should I do next?
Results are typically available within 1–3 business days. Your doctor will discuss them with you and may recommend further testing or treatments based on the findings.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy