Skip to content

Follicle Stimulating Hormone (FSH) with Triple Stimulation Protocol (Serum)

Published: August 9, 2026Updated: August 16, 2026

This blood test measures Follicle Stimulating Hormone (FSH) levels after a controlled stimulation process to evaluate reproductive hormone function.

Quick summary

["Assesses how your body responds to hormone stimulation for fertility or hormonal health evaluation."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
40363-4
Short code
FSH 2h p Triple Bolus SerPl-aCnc
Synonyms
FSH 2h p Triple Bolus SerPl-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.8
Ref high
1.2
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85610
SNOMED
396252006
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test if you're experiencing fertility issues, irregular menstrual cycles, or symptoms related to hormonal imbalances. It helps determine whether your ovaries or testes are responding normally to hormonal signals.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the blood draw. Bring any medications you’re currently taking to your appointment.

What Happens During the Test

A healthcare provider will collect a blood sample from a vein in your arm. The sample is sent to a lab for analysis after your triple-stimulation protocol (typically involving hormone injections over days).

What the Results Mean

Results compare your FSH levels before and after stimulation to check if your reproductive system is functioning as expected. Abnormal results may indicate conditions like premature ovarian insufficiency or hypogonadism.

Reference range

INR: 0.8-1.2 (warfarin: 2.0-3.0)N/A

Specimen: serum

Inform your healthcare provider if you are taking blood thinners. No special preparation required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: This test helps diagnose or monitor conditions affecting hormone regulation, fertility, and reproductive health. Elevated or low FSH levels post-stimulation can signal underlying issues requiring further medical evaluation.

Short name

FSH Triple Stimulation Test

Frequently asked questions

Why was this test ordered for me?
Your doctor may have recommended it to assess reproductive function, fertility potential, or hormonal imbalances—especially if you’re undergoing fertility treatment or experiencing irregular cycles.
What does a ‘triple stimulation’ mean in this test?
It means your body is exposed to controlled doses of hormones (like FSH) over days to observe how your reproductive system reacts. This helps doctors evaluate responsiveness beyond just baseline levels.
Will I feel any side effects from the blood draw?
You may feel slight discomfort or bruising at the injection site, but most people experience minimal pain. Avoid strenuous activity for 24 hours afterward.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy