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Follicle-stimulating hormone (FSH) with triple bolus stimulation testing (serum/plasma)

Published: August 9, 2026Updated: August 16, 2026

This blood test measures your body’s response to a hormone stimulation challenge to evaluate fertility and ovarian function.

Quick summary

["Assesses how well your ovaries react to hormone signals for fertility planning."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
40364-2
Short code
FSH 30M p Triple Bolus SerPl-aCnc
Synonyms
FSH 30M p Triple Bolus SerPl-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.8
Ref high
1.2
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85610
SNOMED
396252006
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Your doctor will administer a hormone (follicle-stimulating hormone, or FSH) through a series of injections over a few days. Blood samples will be taken before and after these injections to track hormone levels and ovarian activity. This helps determine if your ovaries are responsive to stimulation, which is important for fertility treatments.

Why It’s Done

This test is often used in women who are trying to conceive but have irregular periods, low ovarian reserve, or unexplained infertility. It may also be part of a broader evaluation for conditions like polycystic ovary syndrome (PCOS).

How the Results Are Used

Results help doctors understand how well your ovaries produce eggs in response to stimulation. This information guides decisions about fertility treatments, such as in vitro fertilization (IVF) or other hormone therapies.

What to Expect Before the Test

You may need to avoid certain medications (like birth control) or have a baseline blood test before starting the stimulation phase. Follow your doctor’s instructions carefully to ensure accurate results.

Reference range

INR: 0.8-1.2 (warfarin: 2.0-3.0)N/A

Specimen: serum

Inform your healthcare provider if you are taking blood thinners. No special preparation required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

FSH Stimulation Test

Frequently asked questions

How long will the test take?
The test involves multiple blood draws over several days, typically lasting about 1–2 weeks. Your doctor will schedule appointments to collect samples before, during, and after hormone injections.
Will I feel any side effects from the hormone injections?
Some women may experience mild side effects like bloating, breast tenderness, or mood changes during hormone stimulation. These are usually temporary and manageable with support from your healthcare team.
Can I still exercise or eat normally during the test?
Yes, you can generally continue your normal activities unless your doctor advises otherwise. Avoid alcohol and certain medications that could interfere with hormone levels.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy