Fungal 18S rRNA Gene Sequencing (from Stool)

This test helps your doctor find out whether certain conditions may be affecting your health. It can confirm or rule out a possible diagnosis, guide treatment decisions, and give a clearer picture of how your body is functioning so you and your care team can plan the next steps together.

Your healthcare provider may recommend this test if you have symptoms like persistent diarrhea, abdominal pain, or unexplained weight loss that could suggest a fungal infection. It’s also used in research or specialized care for patients with suspected fungal gastrointestinal infections, such as those with weakened immune systems or chronic conditions like Crohn’s disease.

Before your test, your care team will explain the procedure and answer any questions you have. You may be asked to avoid eating or drinking for several hours, pause certain medications, or wear comfortable clothing. Be sure to mention any allergies, existing conditions, or medicines you take, including supplements.

No special preparation is needed, but you may be asked to fast for 8 hours before the test if stool is collected at home. If done in a lab, you’ll likely provide a fresh stool sample. Avoid using laxatives or enemas unless instructed.

During the test, a trained technician or nurse collects the needed sample or takes the measurements using sterile, standard equipment. The process is usually quick and causes little discomfort. The collected material or data is then sent to a laboratory or analyzed on site using established methods.

A small sample of your stool is collected and sent to a lab. The lab extracts genetic material (DNA) from fungi present in the sample. Specialized sequencing technology reads the 18S rRNA gene, a region unique to fungi, to identify the species causing the infection.

Your results show whether the values or findings fall within the normal range for someone of your age and health status. A normal result usually means no sign of the condition being tested, while an abnormal result may suggest that further evaluation is needed. Always review your results with your doctor.

Results show the presence and types of fungal species detected. Positive results indicate a fungal infection, while negative results mean no detectable fungi were found in this sample. Interpretation depends on clinical symptoms and other test results.