Skip to content

Total Protein with Albumin and Globulin Fractionation (Serum)

Published: August 9, 2026Updated: August 16, 2026

This blood test measures your total protein levels and breaks down proteins into albumin and globulin fractions to help assess liver function, nutritional status, and potential protein-related health issues.

Quick summary

This test helps identify protein imbalances that may signal liver disease, malnutrition, or other conditions.

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
77725-0
Short code
FVIII+vWf:RCo Gvn Dose vWF dose-aCnc
Synonyms
FVIII+vWf:RCo Gvn Dose vWF dose-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
vwf-dosing
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test to check for conditions like liver disease (e.g., cirrhosis), kidney problems, malnutrition, or infections that affect protein production. It can also help monitor treatment responses for certain conditions.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if albumin is being measured separately. Bring any medications you’re taking to your appointment.

What the Results Mean

Total protein levels and albumin/globulin ratios can indicate whether your body is producing enough proteins or if there’s an issue with how proteins are used in your body. Abnormal results may prompt further testing.

Common Conditions Monitored

Low total protein or albumin may signal liver disease, kidney failure, or severe malnutrition. High globulin levels could suggest chronic infections, autoimmune disorders, or other inflammatory conditions.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test plays a key role in diagnosing and managing conditions affecting protein metabolism, such as liver cirrhosis, nephrotic syndrome, or protein-losing gastrointestinal disorders. It’s also used to assess nutritional status in patients with chronic illnesses.

Short name

Protein profile test

Frequently asked questions

What does a low total protein level mean?
A low total protein level may indicate liver disease, kidney problems, or malnutrition. Your doctor will review your results alongside other symptoms and tests to determine the cause.
How long does it take to get my results?
Results are typically available within 1–3 business days, depending on your lab. Follow-up with your healthcare provider to discuss findings.
Can I eat or drink before this test?
For most protein tests, fasting isn’t required, but if albumin is being measured separately, avoid food/drinks for 8–12 hours before drawing blood.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy