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Serum or plasma concentration of free fatty acids (via enzymatic method)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free fatty acids in your blood, which are important energy sources for your body.

Quick summary

["Free fatty acids help fuel your cells and are closely monitored in conditions like diabetes or metabolic disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
33121-5
Short code
GA from OFD.Lessoway98
Synonyms
GA from OFD.Lessoway98
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
us-estimated-from-ofd-lessoway
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Are Free Fatty Acids?

Free fatty acids are a type of fat that circulate in your bloodstream. They are released from fat cells when your body needs energy, especially during fasting or exercise. Your liver also produces them. These fats are essential for energy but can become problematic if levels are too high or too low.

Why Might This Test Be Ordered?

Your healthcare provider may order this test if you have symptoms like fatigue, muscle weakness, or if you have conditions such as diabetes, thyroid disorders, or metabolic syndrome. It may also be checked before certain treatments, like chemotherapy, or to monitor long-term health in people with chronic illnesses.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if instructed. Bring any medications you’re taking, as some can affect results. Wear comfortable clothing and plan to stay near the testing site for a few minutes.

What Happens During the Test?

A healthcare professional will draw blood from a vein in your arm using a small needle. You may feel a brief pinch, but it usually doesn’t cause significant discomfort. The blood sample is then sent to a lab for analysis.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Free Fatty Acids (FFA)

Frequently asked questions

How long does it take to get results?
Results are typically available within 1–3 business days, depending on your lab’s processing time. Your healthcare provider will discuss the results with you during your follow-up visit.
Can diet or exercise affect my FFA levels?
Yes, diet and exercise can influence free fatty acid levels. Fasting, high-fat meals, or intense exercise may temporarily raise them, while a balanced diet or moderate activity can help regulate them. Always follow your provider’s advice on timing your test.
Do I need to avoid certain foods before this test?
No specific foods are restricted, but if fasting is required, avoid eating or drinking anything except water for the specified period. If you’re unsure, ask your provider for exact instructions tailored to your situation.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy