Serum Protein Electrophoresis with Bromocresol Green (BCG) Dye Binding
This test measures the distribution of different protein types in your blood serum using electrophoresis and a dye that binds to specific proteins.
["Helps identify abnormal protein patterns, often linked to conditions like multiple myeloma or other blood disorders."]
Key facts at a glance
- LOINC
- 54391-8
- Short code
- Gamma glob MFr Dial fld Elph
- Synonyms
- Gamma glob MFr Dial fld Elph
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 7.35
- Ref high
- 7.45
- Critical range
- 7.2 N/A – 7.6 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- electrophoresis
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
Electrophoresis separates blood proteins based on size and charge, creating bands that reflect the types and amounts of proteins present. The BCG dye binds to albumin and globulins, helping distinguish between them. This process can reveal shifts in protein composition that may signal underlying health issues.
Why It’s Done
Your healthcare provider may order this test to diagnose or monitor conditions affecting protein levels, such as kidney disease, liver disorders, or certain cancers. It’s also used to screen for conditions like multiple myeloma, where abnormal protein production occurs.
Sample Collection and Preparation
A blood sample is drawn from a vein, typically in your arm. The serum (liquid part of blood after clotting) is then prepared for electrophoresis. The BCG dye is added to detect protein concentrations during the separation process.
What to Expect Before the Test
No special preparation is usually needed, but you may be asked to avoid certain medications or foods that could interfere with results. Fasted or non-fasted status may not affect this test.
Reference range
NCBI: pH 7.35-7.45N/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Abnormal protein patterns detected in this test can indicate conditions like multiple myeloma (where abnormal proteins like monoclonal gammas are produced), chronic liver disease, or kidney problems. Low albumin levels may suggest malnutrition or liver dysfunction, while elevated globulins could point to infections or autoimmune disorders.
Short name
Protein Electrophoresis (BCG)
Frequently asked questions
What does a high or low protein band mean?
How long does it take to get results?
Can diet or medications affect this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy