Serum Protein Electrophoresis (SPE) with Bromocresol Green Staining

Why This Test Is Done

Your healthcare provider may order this test if they suspect conditions affecting protein levels, such as kidney disease, liver problems, or certain cancers. It can also be used to monitor treatment progress in conditions like multiple myeloma or to check for signs of inflammation or infection.

How the Test Works

A small blood sample is taken and mixed with a special dye (bromocresol green). The sample is then placed on a gel and subjected to an electric current, causing proteins to separate based on size and charge. The dye highlights different protein bands, which are then analyzed to determine their relative amounts.

Sample Preparation and Collection

You will typically fast for 8–12 hours before the test, though this may vary. Your blood is drawn from a vein in your arm. If you have a clotting disorder or are on blood thinners, let your provider know to avoid complications.

Interpreting Results

Results show protein bands across the gel, representing different protein groups (e.g., albumin, globulins). Abnormal patterns may indicate conditions like hypoalbuminemia, monoclonal gammopathy, or immune system disorders. Your provider will discuss findings with you based on your symptoms and medical history.