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Triple bolus insulin sensitivity test (serum/plasma glucose and insulin measurements)

Published: August 9, 2026Updated: August 16, 2026

This test evaluates how your body responds to rapid insulin doses, helping assess insulin sensitivity and glucose metabolism.

Quick summary

["Measures how insulin affects blood sugar levels after quick injections."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
40338-6
Short code
GH 1h p Triple Bolus SerPl-mCnc
Synonyms
GH 1h p Triple Bolus SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0.8
Ref high
1.2
Critical range
— – 5 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
85610
SNOMED
396252006
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test involves multiple injections of insulin followed by blood draws to measure glucose and insulin levels at specific times. It helps determine how efficiently your body uses insulin to control blood sugar, which is critical for diagnosing or monitoring conditions like diabetes or insulin resistance.

How the Test Works

You’ll receive insulin doses quickly (a 'bolus'), then blood samples are taken to check glucose and insulin levels at set intervals. This helps doctors see how your body reacts to insulin over time.

Why It’s Done

Doctors may order this test if you have symptoms of insulin resistance, unexplained blood sugar fluctuations, or if you’re being evaluated for conditions like prediabetes or type 2 diabetes.

Preparation Tips

No special preparation is needed, but avoid eating or drinking for at least 8 hours before the test. Wear comfortable clothing for multiple injections.

Reference range

INR: 0.8-1.2 (warfarin: 2.0-3.0)N/A

Specimen: serum

Inform your healthcare provider if you are taking blood thinners. No special preparation required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test provides critical insights into insulin sensitivity, aiding in the diagnosis or management of metabolic disorders. It helps tailor treatment plans, such as adjusting insulin therapy or lifestyle changes, to improve blood sugar control.

Frequently asked questions

What does a triple bolus insulin sensitivity test involve?
It involves receiving rapid insulin doses (boluses) followed by blood tests to measure glucose and insulin levels at different times to assess how your body responds to insulin.
How long does the test take?
The test typically takes about 2 to 4 hours, depending on the number of blood draws and insulin doses administered.
Can I eat or drink during the test?
No, you should fast for at least 8 hours before the test to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy