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Serum Iron and Folic Acid Levels

Published: August 9, 2026Updated: August 17, 2026

This test measures your body’s iron stores and vitamin B12 (folic acid) levels to check for deficiencies that could affect energy, blood health, or pregnancy.

Quick summary

["Iron and folic acid deficiencies are common and can cause fatigue, weakness, or other symptoms—this test helps identify them early."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
72180-3
Short code
GIVENIRONANDFOLICACID
Synonyms
—
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
60
Ref high
170
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
rhea
CPT
83540
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why These Tests Matter

Iron is essential for making hemoglobin, the protein in red blood cells that carries oxygen throughout your body. Low iron can lead to anemia, which may cause fatigue, pale skin, or shortness of breath. Folic acid is a B vitamin that helps your body produce healthy red blood cells and DNA. Deficiencies can also lead to anemia and other health issues, especially during pregnancy.

What to Expect Before the Test

You’ll likely have blood drawn from a vein in your arm. No special preparation is needed, but inform your doctor if you’re taking iron supplements or have a bleeding disorder. Fasting is not required for either test.

Common Causes of Low Levels

Iron deficiency often results from poor diet, heavy menstrual bleeding, pregnancy, or chronic blood loss. Folic acid deficiency may stem from diet low in leafy greens, alcohol use, or certain medications. Both deficiencies can also occur if your body doesn’t absorb nutrients properly.

Symptoms to Watch For

Fatigue, pale skin, weakness, or unusual tiredness are common signs of low iron or folic acid. Other symptoms may include headaches, dizziness, or a sore tongue. If you experience these, talk to your doctor about testing.

Reference range

NCBI: Serum Iron Male 65-180; Female 50-170 µg/dLN/A

Specimen: serum

Fasting for 12 hours preferred. Iron supplements should be stopped 24 hours before if possible.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Iron & Folic Acid

Frequently asked questions

What Does a Low Level Mean?
Low iron or folic acid levels may indicate anemia, pregnancy complications, or other health conditions like celiac disease or malabsorption disorders. High levels (in iron) could signal iron overload, which can be dangerous if untreated.
How Often Should I Get Tested?
Your doctor may recommend testing if you have symptoms of deficiency, are pregnant, have a history of anemia, or are at risk for nutrient deficiencies (e.g., poor diet, chronic illness). Regular check-ups can help catch issues early.
Can I Prevent Deficiencies?
Yes! Eat iron-rich foods like lean meats, spinach, and fortified cereals, and folic acid-rich foods like beans, nuts, and leafy greens. If you’re pregnant, your doctor may recommend supplements. Avoid excessive alcohol, which can interfere with folic acid absorption.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy