Serum Iron and Folic Acid Levels
This test measures your body’s iron stores and vitamin B12 (folic acid) levels to check for deficiencies that could affect energy, blood health, or pregnancy.
["Iron and folic acid deficiencies are common and can cause fatigue, weakness, or other symptoms—this test helps identify them early."]
Key facts at a glance
- LOINC
- 72180-3
- Short code
- GIVENIRONANDFOLICACID
- Synonyms
- —
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 60
- Ref high
- 170
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- rhea
- CPT
- 83540
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why These Tests Matter
Iron is essential for making hemoglobin, the protein in red blood cells that carries oxygen throughout your body. Low iron can lead to anemia, which may cause fatigue, pale skin, or shortness of breath. Folic acid is a B vitamin that helps your body produce healthy red blood cells and DNA. Deficiencies can also lead to anemia and other health issues, especially during pregnancy.
What to Expect Before the Test
You’ll likely have blood drawn from a vein in your arm. No special preparation is needed, but inform your doctor if you’re taking iron supplements or have a bleeding disorder. Fasting is not required for either test.
Common Causes of Low Levels
Iron deficiency often results from poor diet, heavy menstrual bleeding, pregnancy, or chronic blood loss. Folic acid deficiency may stem from diet low in leafy greens, alcohol use, or certain medications. Both deficiencies can also occur if your body doesn’t absorb nutrients properly.
Symptoms to Watch For
Fatigue, pale skin, weakness, or unusual tiredness are common signs of low iron or folic acid. Other symptoms may include headaches, dizziness, or a sore tongue. If you experience these, talk to your doctor about testing.
Reference range
NCBI: Serum Iron Male 65-180; Female 50-170 µg/dLN/A
Fasting for 12 hours preferred. Iron supplements should be stopped 24 hours before if possible.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
Iron & Folic Acid
Frequently asked questions
What Does a Low Level Mean?
How Often Should I Get Tested?
Can I Prevent Deficiencies?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy