Serum Protein Electrophoresis (Bromocresol Purple Method)
This test measures the distribution of different protein types in your blood serum using electrophoresis, often with a dye like bromocresol purple to visualize bands. It helps identify patterns of protein concentration that may indicate underlying health conditions.
["This test helps doctors assess protein balance and spot potential issues like infections, liver disease, or kidney problems."]
Key facts at a glance
- LOINC
- 12217-6
- Short code
- Globulin Fld-mCnc
- Synonyms
- Globulin Fld-mCnc
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
Electrophoresis separates blood proteins into distinct bands based on size and charge. The bromocresol purple dye binds to proteins, creating visible bands that reflect the concentration of each protein group (e.g., albumin, globulins, fibrinogen). This pattern can reveal abnormalities in protein distribution.
Why It’s Done
Your doctor may order this test if you have symptoms like fatigue, swelling, or unexplained weight changes, or if they suspect conditions like multiple myeloma, chronic inflammation, or liver/kidney disease. It’s also used to monitor treatment progress for certain disorders.
Sample Collection and Preparation
A blood sample is drawn from a vein, usually in the arm. The serum (liquid part after clotting) is then separated and analyzed using electrophoresis. The dye reaction creates bands that are measured for concentration.
Common Conditions Evaluated
This test helps diagnose or monitor conditions like chronic inflammation (e.g., rheumatoid arthritis), infections, liver disease, kidney disease, or certain cancers (e.g., multiple myeloma). Abnormal patterns may indicate imbalances in protein types.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Abnormal electrophoresis results can signal underlying health issues. For example, a shift toward higher globulin levels might suggest chronic infection or autoimmune disease, while low albumin could indicate liver or kidney problems. Always discuss results with your healthcare provider.
Short name
Protein Electrophoresis
Frequently asked questions
What does an abnormal protein electrophoresis result mean?
How often should I get this test?
Can diet affect protein electrophoresis results?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy