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Glucose (Serum) with High-Performance Liquid Chromatography (HPLC) Measurement

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of glucose (sugar) in your blood serum using advanced chromatography. It helps assess blood sugar levels and identify potential issues like diabetes or metabolic disorders.

Quick summary

["This test helps monitor blood sugar control and detect hidden sugar-related problems."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
20440-4
Short code
Glucose 1.5h p Glc SerPl-mCnc
Synonyms
Glucose 1.5h p Glc SerPl-mCnc
Category
glucose
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
70
Ref high
100
Critical range
50 N/A – 400 N/A
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
CPT
82947
SNOMED
260395008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test to check for conditions like diabetes, hypoglycemia, or other metabolic disorders. It may also be used during pregnancy to monitor gestational diabetes or to evaluate how your body processes glucose over time.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before testing if glucose levels are being specifically monitored. Bring any medications you’re taking to your appointment.

How the Test Works

A blood sample is drawn from a vein, usually in your arm. The serum portion is then analyzed using high-performance liquid chromatography to precisely measure glucose concentration.

What the Results Mean

Results provide a detailed glucose profile, which may include baseline levels and trends over time. While reference ranges vary by lab, abnormal results may signal elevated or low blood sugar, warranting further evaluation.

Reference range

Fasting: 70-100 mg/dLN/A

Specimen: serum

Fasting for 8-12 hours required. Water is permitted. Avoid chewing gum and smoking before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Serum Glucose Profile

Frequently asked questions

How often should I get this test?
The frequency depends on your health status. People with diabetes typically check glucose levels monthly or more often, while others may need testing less frequently unless symptoms arise.
What if my results are outside the normal range?
If results are abnormal, your doctor may recommend additional tests (e.g., HbA1c) or lifestyle adjustments. Never interpret results independently—always discuss findings with a healthcare provider.
Does fasting affect this test?
Yes, fasting may alter glucose readings. If fasting is required, follow your provider’s instructions to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy