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Glucose (Serum) – High-Performance Liquid Chromatography (HPLC) Method

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of glucose, a type of sugar, in your blood serum using a specialized chromatography technique. Glucose is the primary energy source for your body’s cells.

Quick summary

["This test helps monitor blood sugar levels, especially important for managing diabetes or other conditions affecting glucose metabolism."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25664-4
Short code
Glucose 1h p Glc Ur-mCnc
Synonyms
Glucose 1h p Glc Ur-mCnc
Category
glucose
Sample
urine
Container
Sterile urine container
Units
N/A
Ref low
70
Ref high
100
Critical range
50 N/A – 400 N/A
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
CPT
82947
SNOMED
260395008
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test to check for diabetes, monitor blood sugar levels over time, or evaluate how your body processes glucose. It’s also used in research or specialized diagnostic settings where precise glucose measurement is critical.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if it’s part of a fasting glucose profile. Bring any medications you’re taking to your appointment.

How the Test Works

A blood sample is drawn from a vein, usually in your arm. The serum portion is separated and analyzed using high-performance liquid chromatography to detect and quantify glucose accurately.

What the Results Mean

Results are reported as a mass concentration of glucose in your serum. While reference ranges vary by lab, abnormal results may signal conditions like hypoglycemia (low glucose) or hyperglycemia (high glucose).

Reference range

Fasting: 70-100 mg/dLN/A

Specimen: urine

Fasting for 8-12 hours required. Water is permitted. Avoid chewing gum and smoking before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Glucose Level (Serum)

Frequently asked questions

What causes abnormal glucose levels?
Abnormal glucose results can be caused by diet, physical activity, certain medications, or underlying conditions like diabetes. If you notice frequent fluctuations, discuss them with your provider.
How often should I get this test?
Your provider will determine the frequency based on your health needs. Regular monitoring may be recommended if you have diabetes, prediabetes, or other metabolic conditions.
Can I eat or drink before this test?
If fasting is required, avoid food and liquids (except water) for the specified period. Follow your provider’s instructions to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy