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Glucose measured by high-performance liquid chromatography (HPLC) in serum/plasma

Published: August 9, 2026Updated: August 16, 2026

This test measures your blood sugar level using a specialized HPLC method, which provides precise glucose concentration from a blood sample.

Quick summary

["HPLC glucose testing offers highly accurate blood sugar readings for monitoring diabetes or other metabolic conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
20436-2
Short code
Glucose 2h p Glc SerPl-mCnc
Synonyms
Glucose 2h p Glc SerPl-mCnc
Category
glucose
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
70
Ref high
100
Critical range
50 N/A – 400 N/A
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
CPT
82947
SNOMED
260395008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test to diagnose or monitor conditions like diabetes, hypoglycemia, or metabolic disorders. It helps track how well your body manages glucose over time.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in your arm. The sample is processed to separate serum or plasma for accurate glucose measurement via HPLC, which separates and detects glucose molecules.

How Results Are Interpreted

Results are expressed as a mass concentration of glucose in your blood. While reference ranges vary by lab, they generally fall within a specific range for healthy individuals, though exact values depend on your condition and medical history.

Common Reasons for Testing

This test is often used in routine diabetes management, post-meal monitoring, or during investigations for unexplained symptoms like fatigue, frequent urination, or unexplained weight changes.

Reference range

Fasting: 70-100 mg/dLN/A

Specimen: serum

Fasting for 8-12 hours required. Water is permitted. Avoid chewing gum and smoking before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Glucose Level (HPLC)

Frequently asked questions

What Does a High or Low Glucose Level Mean?
A high glucose level may suggest diabetes, poor insulin function, or other metabolic disorders. A low glucose level could indicate hypoglycemia, especially if symptoms like shakiness or dizziness occur.
How Often Should I Get This Test?
Frequency depends on your medical condition. For example, people with diabetes may need testing every few months or more often if managing complex care plans.
Can I Fast Before This Test?
Yes, fasting is often required (typically 8–12 hours) to get an accurate baseline glucose reading, especially if monitoring diabetes or metabolic health.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy