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Glucose (Serum) with High-Performance Liquid Chromatography (HPLC) Method

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of glucose (sugar) in your blood serum using a precise liquid chromatography technique. It helps assess how well your body is managing blood sugar levels.

Quick summary

["This test helps monitor blood sugar control, often used in diabetes management or routine health checks."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
26555-3
Short code
Glucose 3.5h p Glc SerPl-mCnc
Synonyms
Glucose 3.5h p Glc SerPl-mCnc
Category
glucose
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
70
Ref high
100
Critical range
50 N/A – 400 N/A
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
CPT
82947
SNOMED
260395008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test to check for conditions like diabetes, hypoglycemia (low blood sugar), or to monitor existing diabetes management. It may also be part of a general health evaluation or screening for metabolic disorders.

How the Test Works

A blood sample is taken from a vein, usually in your arm. The sample is processed to separate serum (the liquid part after blood clotting) and analyzed using HPLC to detect glucose levels with high accuracy.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast (not eat) for 8–12 hours before the test if it’s part of a fasting glucose profile. Let your provider know about any medications or recent illnesses.

What the Results Mean

Results provide a precise measurement of glucose concentration in your serum. While reference ranges vary by lab, abnormal results may signal high or low blood sugar, prompting further evaluation.

Reference range

Fasting: 70-100 mg/dLN/A

Specimen: serum

Fasting for 8-12 hours required. Water is permitted. Avoid chewing gum and smoking before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Glucose Level Test

Frequently asked questions

What causes false high or low results?
False results may occur if you didn’t fast properly, had recent exercise, or took certain medications. Lab errors or sample contamination can also affect accuracy. Always discuss your results with your provider.
How often should I get this test?
Frequency depends on your health status. People with diabetes typically check glucose levels monthly or more often as directed by their doctor. Routine adults may get it less frequently unless advised otherwise.
Can I eat or drink before this test?
If fasting is required, avoid food and liquids (except water) for 8–12 hours before the test. If no fasting is needed, eat normally. Ask your provider for exact instructions tailored to your situation.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy