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Glucose measured in plasma (3 AM)

Published: August 9, 2026Updated: August 16, 2026

This test checks your blood sugar level in plasma collected just before or at 3 AM, often done to assess how your body manages glucose overnight.

Quick summary

["A simple check of your blood sugar levels during a specific overnight period."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
48993-0
Short code
Glucose 3 AM SerPl-mCnc
Synonyms
Glucose 3 AM SerPl-mCnc
Category
glucose
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
70
Ref high
100
Critical range
50 N/A – 400 N/A
Fasting
true
Population
adult
Sex
all
Age range
18–65 years
CPT
82947
SNOMED
260395008
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order this test to evaluate how your body handles glucose during sleep, especially in people with suspected or known diabetes, metabolic disorders, or those undergoing certain medical evaluations. It helps assess overnight glucose fluctuations that standard fasting tests may miss.

What to Expect Before the Test

You’ll typically fast for 8–12 hours before the test. Avoid eating, drinking (except water), smoking, or taking medications unless instructed otherwise. Your blood will be drawn from a vein in your arm by a healthcare professional.

What the Test Results Mean

This test measures the concentration of glucose (sugar) in your plasma at a specific time of night. Results help identify patterns in your blood sugar, such as whether your body produces enough insulin or if there’s an issue with how your body uses glucose.

Common Causes for Testing

This test may be recommended if you have symptoms like frequent urination, excessive thirst, unexplained weight changes, fatigue, or if you’re at risk for diabetes. It’s also used in research or clinical studies to monitor overnight glucose trends.

Reference range

Fasting: 70-100 mg/dLN/A

Specimen: serum

Fasting for 8-12 hours required. Water is permitted. Avoid chewing gum and smoking before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Morning plasma glucose

Frequently asked questions

How is this test different from a standard fasting glucose test?
Unlike fasting glucose tests, which check blood sugar after an overnight fast, this test specifically measures glucose at 3 AM. It helps detect subtle overnight fluctuations that could indicate conditions like hypoglycemia (low blood sugar), diabetes, or other metabolic issues.
What if my results are outside of normal ranges?
If your results are higher or lower than expected, your healthcare provider will discuss them with you. They may recommend further testing, lifestyle changes, or adjustments to medications to manage your blood sugar effectively.
Can I eat or drink anything before this test?
No, you must fast for 8–12 hours before the test. Only water is allowed. Follow your healthcare provider’s instructions to ensure accurate results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy