Glucose (Serum) with 24-Hour Profile Differentiation by High-Performance Liquid Chromatography (HPLC)
This test measures glucose levels in your blood over a 24-hour period, using a specialized HPLC method to distinguish variations in blood sugar.
["A glucose profile helps track how your blood sugar fluctuates throughout the day, useful for managing conditions like diabetes."]
Key facts at a glance
- LOINC
- 59797-1
- Short code
- Glucose 5h p chal SerPl-SCDiff
- Synonyms
- Glucose 5h p chal SerPl-SCDiff
- Category
- glucose
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 70
- Ref high
- 100
- Critical range
- 50 N/A – 400 N/A
- Fasting
- true
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 82947
- SNOMED
- 260395008
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Your healthcare provider may order this test if you have diabetes, prediabetes, or symptoms like frequent urination, excessive thirst, or unexplained weight changes. It may also be used to assess how well your body responds to glucose over time, especially if you’re taking medications that affect blood sugar levels.
What to Expect Before the Test
No special preparation is typically needed, but you may be asked to fast for a portion of the 24-hour period (e.g., overnight fasting). Follow your provider’s instructions carefully to ensure accurate results. Bring any medications you’re taking to discuss with your doctor.
During the Test Process
Blood samples will be collected at multiple intervals (e.g., fasting and post-meal) over 24 hours. A healthcare professional will draw blood from a vein, usually in your arm. The samples are processed using HPLC to analyze glucose at different times.
What the Results Mean
The results show your glucose levels at various points, helping identify patterns like spikes after meals or low levels during certain times. Abnormal patterns may indicate poor blood sugar control, insulin resistance, or other metabolic issues.
Reference range
Fasting: 70-100 mg/dLN/A
Fasting for 8-12 hours required. Water is permitted. Avoid chewing gum and smoking before the test.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
Glucose Profile (24h HPLC)
Frequently asked questions
This test is critical for diagnosing and monitoring diabetes, prediabetes, or hypoglycemia. It provides a more detailed picture than a single blood glucose check, helping doctors tailor treatment plans (e.g., medication adjustments, lifestyle changes).
Blood samples are collected at home or in a clinic, depending on your provider’s instructions. If you’re collecting samples at home, ensure you follow sterile techniques to avoid contamination.
The HPLC method ensures precise glucose measurements, but results may still be affected by lab errors or specimen handling. Report any issues (e.g., missed samples) to your provider immediately.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy