Line Blot Immunoassay for Antibodies to Borrelia burgdorferi (Lyme Disease Antibody Screening)

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you’ve had symptoms like fever, fatigue, joint pain, or a rash (e.g., erythema migrans) after a tick bite, especially in areas where Lyme disease is common. It can also be used as part of a broader evaluation for unexplained infections or autoimmune concerns.

What to Expect Before the Test

No special preparation is needed. You’ll have a small needle inserted to draw blood from a vein, usually in your arm. The process takes about 5 minutes, and you may feel a brief pinch. If you’re pregnant, have a bleeding disorder, or take blood-thinning medications, let your provider know beforehand.

What the Test Results Mean

Results are qualitative (positive/negative) and not used alone to diagnose Lyme disease. A positive result suggests possible exposure but doesn’t confirm infection. Your provider will likely order additional tests (e.g., ELISA or Western blot) for confirmation.

Common Symptoms Linked to Lyme Disease

Lyme disease often starts with flu-like symptoms (fever, chills, fatigue) followed by a bullseye rash (erythema migrans) at the bite site. Later stages may include joint pain, neurological issues (e.g., meningitis), or heart problems. Symptoms can vary widely and may not appear for weeks or months after exposure.