Vitamin D [25-hydroxy] in Serum
This test measures the level of vitamin D in your blood, specifically the form that your body converts from sunlight exposure or diet to use for bone health and other functions.
["Vitamin D levels help ensure your bones stay strong and healthy."]
Key facts at a glance
- LOINC
- 581-9
- Short code
- Gp B Strep Cvx Ql Cult
- Synonyms
- Gp B Strep Cvx Ql Cult
- Category
- coagulation
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- organism-specific-culture
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why Is This Test Done?
Your doctor may order this test to check if you have enough vitamin D, which is important for preventing conditions like rickets in children or osteoporosis in adults. It can also help diagnose or monitor vitamin D deficiency, which may cause fatigue, muscle weakness, or bone pain. Sometimes, it’s used to assess vitamin D levels in people with certain medical conditions, like kidney disease or malabsorption disorders.
What Does the Test Involve?
A blood sample is taken from a vein in your arm. You may feel a small pinch when the needle goes in, but it usually doesn’t cause discomfort afterward. The blood sample is then sent to a lab for testing.
What to Expect Before or After the Test
There are no special preparations needed for this test. You can eat, drink, or take your usual medications as usual. If you’re pregnant or have a medical condition, let your doctor know before the test. After the blood is drawn, you can resume your normal activities immediately.
What Are Common Causes of Low or High Levels?
Low levels of vitamin D can result from insufficient sun exposure, poor diet, certain medications, or conditions that affect how your body absorbs nutrients. High levels may occur if you take too much vitamin D supplement or have certain kidney or liver diseases.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
25-Hydroxy Vitamin D (Serum)
Frequently asked questions
How long does it take to get the results of this test?
What do my vitamin D test results mean?
Can I take vitamin D supplements to increase my levels?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy