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Vitamin D [25-hydroxy] in Serum

Published: August 9, 2026Updated: August 16, 2026

This test measures the level of vitamin D in your blood, specifically the form that your body converts from sunlight exposure or diet to use for bone health and other functions.

Quick summary

["Vitamin D levels help ensure your bones stay strong and healthy."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
581-9
Short code
Gp B Strep Cvx Ql Cult
Synonyms
Gp B Strep Cvx Ql Cult
Category
coagulation
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
organism-specific-culture
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your doctor may order this test to check if you have enough vitamin D, which is important for preventing conditions like rickets in children or osteoporosis in adults. It can also help diagnose or monitor vitamin D deficiency, which may cause fatigue, muscle weakness, or bone pain. Sometimes, it’s used to assess vitamin D levels in people with certain medical conditions, like kidney disease or malabsorption disorders.

What Does the Test Involve?

A blood sample is taken from a vein in your arm. You may feel a small pinch when the needle goes in, but it usually doesn’t cause discomfort afterward. The blood sample is then sent to a lab for testing.

What to Expect Before or After the Test

There are no special preparations needed for this test. You can eat, drink, or take your usual medications as usual. If you’re pregnant or have a medical condition, let your doctor know before the test. After the blood is drawn, you can resume your normal activities immediately.

What Are Common Causes of Low or High Levels?

Low levels of vitamin D can result from insufficient sun exposure, poor diet, certain medications, or conditions that affect how your body absorbs nutrients. High levels may occur if you take too much vitamin D supplement or have certain kidney or liver diseases.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

25-Hydroxy Vitamin D (Serum)

Frequently asked questions

How long does it take to get the results of this test?
Test results are typically available within 1 to 3 business days, depending on your lab’s processing times. Your doctor will contact you with the results once they are ready.
What do my vitamin D test results mean?
Results are interpreted by your healthcare provider based on your overall health, symptoms, and medical history. They will explain whether your levels are within the normal range or if further action is needed, such as dietary changes, supplements, or additional testing.
Can I take vitamin D supplements to increase my levels?
Yes, but only under the guidance of your doctor. They can determine the appropriate dose and monitor your levels to avoid excessive intake, which can be harmful.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy