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Serum or Plasma Protein Electrophoresis (Bromocresol Green)

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of different protein types in your blood, helping identify abnormalities in protein levels that may signal conditions like kidney disease, liver problems, or certain cancers.

Quick summary

["This test helps detect protein imbalances that could indicate underlying health issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
90262-7
Short code
Gram Stn Bifl
Synonyms
Gram Stn Bifl
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
gram-stain
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Proteins Does This Test Measure?

Electrophoresis separates blood proteins based on size and charge, revealing patterns in albumin, globulins (including immunoglobulins), and other proteins. Abnormal bands or shifts may point to conditions like multiple myeloma, Waldenström macroglobulinemia, or chronic kidney disease.

Why Might You Need This Test?

Your doctor may order this test if you have symptoms like unexplained weight loss, fatigue, swelling, or if tests for kidney/liver function or cancer screening suggest protein-related issues. It’s also used in monitoring certain treatments.

How Is the Test Performed?

A blood sample is drawn, and the serum or plasma is placed in a gel or paper strip. An electric current separates proteins, creating distinct bands. Results are compared to normal patterns to identify deviations.

What Do Results Mean?

Normal results show balanced protein bands. Abnormal patterns (e.g., extra bands, low albumin, high globulins) may require further testing or evaluation of your medical history and symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test plays a key role in diagnosing and managing conditions affecting protein metabolism, such as monoclonal gammopathies (e.g., multiple myeloma), liver disease, or nephrotic syndrome. Early detection can guide treatment decisions and improve outcomes.

Short name

Bromocresol Green Protein Electrophoresis

Frequently asked questions

What does a ‘bromocresol green’ method indicate about my test?
The bromocresol green method is a colorimetric technique used to quantify protein concentrations during electrophoresis. It helps assess total protein levels indirectly but isn’t specific to individual proteins. Your results focus on protein band patterns, not exact numeric values.
Can I eat or drink anything before this test?
No special diet is needed, but avoid heavy meals 2 hours before your blood draw. Hydration is fine, but inform your doctor if you’re on certain medications that might affect protein levels.
Will this test show early signs of cancer?
While this test can identify abnormal protein patterns linked to some cancers (e.g., multiple myeloma), it’s not a definitive cancer screen. Results may prompt additional tests, like bone marrow biopsy or other imaging, for further evaluation.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy