Bromocresol Green Protein Electrophoresis (Serum)

What This Test Measures

Electrophoresis separates proteins in your serum based on size and charge. Bromocresol green dye binds to specific protein fractions, allowing for visual or quantitative analysis of these components. The test identifies patterns that may indicate abnormal protein levels or distribution, such as those seen in liver disease, kidney problems, or certain genetic disorders.

Why It’s Done

Your healthcare provider may order this test if you have symptoms like unexplained weight loss, fatigue, swelling, or if they suspect conditions like cirrhosis, nephrotic syndrome, or hereditary protein disorders. It’s also used as part of a broader evaluation for unexplained protein abnormalities in blood tests.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The serum (liquid part of blood after clotting) is then prepared for electrophoresis. The process involves applying the serum to a gel and applying an electric current to separate proteins by size and charge.

What to Expect Before the Test

No special preparation is typically needed, but you may be asked to fast for a few hours before the blood draw if your provider recommends it. Wear comfortable clothing and plan to rest after the blood draw.