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Total Serum Protein by Bromocresol Green (BCG) Method

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of protein found in your blood serum. Proteins help maintain fluid balance, support immune function, and transport nutrients.

Quick summary

["Assesses overall protein levels in your blood for health monitoring or diagnostic purposes."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
87974-2
Short code
Gram Stn Burn
Synonyms
Gram Stn Burn
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
gram-stain
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

The test evaluates the combined weight of all proteins in your blood, including albumin, globulins, and other soluble proteins. This includes both albumin (which helps maintain blood pressure and fluid balance) and globulins (which play roles in immunity and transport).

Why Might You Need This Test?

Your doctor may order this test to check for conditions affecting protein levels, such as kidney disease, liver problems, malnutrition, or certain immune disorders. It can also help monitor treatment responses for conditions like nephrotic syndrome or protein-losing gastrointestinal diseases.

Common Reasons for Testing

Common reasons include evaluating symptoms like swelling (edema), unexplained weight loss, fatigue, or signs of organ dysfunction. It may also be part of routine health checks or follow-up for chronic conditions.

How Is the Test Performed?

A blood sample is drawn from a vein, usually in your arm. The serum portion is separated and tested using the bromocresol green dye method, which binds to proteins and allows measurement of their concentration.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: Interpretation and Implications

Short name

Serum Protein Screening

Frequently asked questions

How are results typically interpreted?
Results are compared to reference ranges for your age and health status. Low protein levels may indicate conditions like malnutrition, liver disease, or kidney problems, while high levels could suggest dehydration, inflammation, or certain cancers.
What does a low or high protein result mean?
A low total protein result may signal underlying health issues requiring further investigation, such as protein-losing conditions or organ dysfunction. A high result may need evaluation for conditions like severe dehydration or chronic inflammation.
Can lifestyle or diet affect this test?
Yes, diet can influence protein levels. High-protein diets or certain medical conditions may temporarily alter results. Avoiding excessive alcohol or certain medications (like diuretics) for a few days before testing can help ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy