Skip to content

Serum or Plasma Total Protein by Bromocresol Green (BCG) Method

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of protein in your blood, which includes albumin and other proteins like globulins. Protein is essential for maintaining healthy tissues and fluids in your body.

Quick summary

["Your blood protein levels help your body stay balanced and support important functions like healing and immune response."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
6464-2
Short code
Gram Stn Cvx
Synonyms
Gram Stn Cvx
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
gram-stain
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may order this test to check for conditions affecting protein levels, such as kidney disease, liver problems, malnutrition, or certain infections. It can also help monitor treatment progress for conditions like protein-losing disorders.

What to Expect Before the Test

No special preparation is usually needed. You may be asked to fast before the test if it’s part of a larger blood work panel, but this isn’t always required for total protein alone. Bring any medications or supplements you’re taking to your appointment.

What Happens During the Test

A healthcare professional will draw blood from a vein, usually in your arm. The blood sample is then sent to a lab for analysis using the bromocresol green dye method to measure protein concentration.

What the Results Mean

Results are typically reported in grams per deciliter (g/dL). Low protein levels may indicate conditions like kidney disease or malnutrition, while very high levels could suggest conditions like multiple myeloma or severe dehydration.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Protein

Frequently asked questions

What causes low protein levels in blood?
Low protein levels can result from conditions like kidney disease, liver problems, malnutrition, or certain infections. It may also occur if your body loses protein through excessive sweating, vomiting, or diarrhea. Lifestyle factors like poor diet or chronic illness can also play a role.
What are the common symptoms of abnormal protein levels?
Symptoms may vary depending on whether protein levels are too high or too low. Low protein levels might cause swelling (edema), fatigue, or frequent infections. High protein levels may not cause noticeable symptoms but can sometimes lead to dehydration or other complications if severe.
How often should I get this test?
Your healthcare provider will determine how often you need this test based on your medical history, symptoms, and overall health. Some people may need it annually or more frequently if they have ongoing health conditions affecting protein levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy