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Guided Immunoassay for Bile Acids (24-Hour Urinary)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of bile acids excreted in your urine over a 24-hour period. Bile acids help digest fats and are produced by the liver.

Quick summary

["This test helps assess liver function and bile flow, often used to diagnose conditions like bile duct blockages or liver disease."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
87015-4
Short code
Guided LN Bx
Synonyms
Guided LN Bx
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
imaging-modality
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may recommend this test if you have symptoms like abdominal pain, unexplained weight loss, or signs of liver or bile duct issues. It can also be used to monitor conditions like cirrhosis, bile duct strictures, or certain genetic disorders affecting bile acid metabolism.

What to Expect Before the Test

You’ll need to collect all urine for 24 hours, starting and ending with the first morning urine. Store the samples in a refrigerator and bring the entire collection to your lab. Follow your provider’s instructions carefully to ensure accurate results.

How the Test Works

The test uses a specialized immunoassay method to detect and quantify bile acids in your urine. The collected urine is processed in the lab to measure the total concentration of these substances over the full day.

What the Results Mean

Elevated levels may indicate problems with bile acid synthesis, liver disease, or blockages in the bile ducts. Lower than expected levels could suggest malabsorption issues or certain genetic disorders. Your provider will interpret results in the context of your overall health and symptoms.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-Hour Urinary Bile Acids

Frequently asked questions

What causes bile acids to be measured in urine over 24 hours?
Bile acids are typically reabsorbed in the intestines, so measuring them in urine helps identify conditions where this reabsorption is impaired, such as liver disease, bile duct obstructions, or certain metabolic disorders. It can also help diagnose conditions like primary biliary cholangitis or cystic fibrosis.
How often should this test be repeated?
Your provider will determine how often to repeat this test based on your symptoms, lab results, and overall health. Some patients may need follow-up testing every few months or annually, depending on their condition.
Can diet or medications affect this test?
Yes, certain foods (like high-fat meals) and medications (such as bile acid sequestrants) can temporarily alter bile acid levels. Your provider will give you specific instructions to follow before the test to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy